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primary human dermal microvascular ecs  (PromoCell)


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    PromoCell primary human dermal microvascular ecs
    Primary Human Dermal Microvascular Ecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 455 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal microvascular ecs/product/PromoCell
    Average 96 stars, based on 455 article reviews
    primary human dermal microvascular ecs - by Bioz Stars, 2026-03
    96/100 stars

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    Image Search Results


    Development of the microfluidic microvascular co-culture model in the PREDICT96 platform. ( A ) Side-view schematic of the microvasculature. Capillaries are surrounded by pericytes which, under healthy conditions, help stabilize and mature the endothelium. ( B ) Cross-section schematic showing interaction of endothelial cells (ECs) and pericytes (PCs) through basement membrane. ( C ) Side-view cross-section schematic of the vascular model in the bilayer microfluidic device. ECs and PCs are cultured on either side of a microporous membrane coated with extracellular matrix, which allows interaction between the two cell types. ( D ) Top-view of the PREDICT96 plate, containing an array of 96 bilayer microfluidic devices that interfaces with a 384 well plate top. ( E ) Schematic of PREDICT96 custom pneumatic pump lid, containing 192 individual pumps that control fluid flow in each channel of the 96 bilayer devices. ( F ) A single PREDICT96 device corresponds to 4 wells of the 384 well plate with architecture allowing for culture and fluid flow in separate top and bottom channels, which overlap in the device center. ( G ) Top-view bright field image of a single device, with channel overlap area indicated as the region of interest (dotted rectangle). ( H ) Representative image of ECs stained for PECAM-1 (green) and Hoechst (blue) in channel overlap area.

    Journal: Scientific Reports

    Article Title: A high-throughput microfluidic bilayer co-culture platform to study endothelial-pericyte interactions

    doi: 10.1038/s41598-021-90833-z

    Figure Lengend Snippet: Development of the microfluidic microvascular co-culture model in the PREDICT96 platform. ( A ) Side-view schematic of the microvasculature. Capillaries are surrounded by pericytes which, under healthy conditions, help stabilize and mature the endothelium. ( B ) Cross-section schematic showing interaction of endothelial cells (ECs) and pericytes (PCs) through basement membrane. ( C ) Side-view cross-section schematic of the vascular model in the bilayer microfluidic device. ECs and PCs are cultured on either side of a microporous membrane coated with extracellular matrix, which allows interaction between the two cell types. ( D ) Top-view of the PREDICT96 plate, containing an array of 96 bilayer microfluidic devices that interfaces with a 384 well plate top. ( E ) Schematic of PREDICT96 custom pneumatic pump lid, containing 192 individual pumps that control fluid flow in each channel of the 96 bilayer devices. ( F ) A single PREDICT96 device corresponds to 4 wells of the 384 well plate with architecture allowing for culture and fluid flow in separate top and bottom channels, which overlap in the device center. ( G ) Top-view bright field image of a single device, with channel overlap area indicated as the region of interest (dotted rectangle). ( H ) Representative image of ECs stained for PECAM-1 (green) and Hoechst (blue) in channel overlap area.

    Article Snippet: Primary human dermal microvascular ECs were purchased from Lonza (Basel, Switzerland) and expanded in the manufacturer’s recommended medium EGM-2MV (EBMTM-2 Basal Medium and Microvascular Endothelial Growth Medium SingleQuotsTM supplements).

    Techniques: Co-Culture Assay, Membrane, Cell Culture, Control, Staining